The Microscopy & Imaging Shared Resource (MISR) provides instrumentation and expertise for light microscopy. Users incorporate a variety of imaging techniques into their studies including total internal reflection microscopy (TIRF), laser scanning and spinning disk confocal, and automated image acquisition for screening of multiwell samples. Applications include fluorescence resonance energy transmission (FRET), fluorescence lifetime intensity microscopy (FLIM) for FRET measurements, fluorescence recovery after photobleaching (FRAP), and other live imaging applications; multispectral emission imaging, and epifluorescence and brightfield time-lapse microscopy, microinjection, immuno-light microscopy and image analysis to study tumor cell biology.
For specific inquiries:
Experimental design, first use discussion: email@example.com
Equipment signup and technical questions: firstname.lastname@example.org
Image analysis and live imaging: email@example.com
- Cytogenetics methods: firstname.lastname@example.org
In all publications that include data derived or methods used from the Microscopy & Imaging Shared Resource, please acknowledge our resource. The Microscopy & Imaging Shared Resource is partially supported by NIH/NCI grant P30-CA051008.
Microscopy & Imaging Shared Resource is on LombardiNet where you can access the MISR intranet site for more information including descriptions of the equipment available, reagents, protocols and much more. A Georgetown University NetID and password are required.