Microscopy & Imaging Shared Resource (MISR)

Contact Information

Michael Johnson, PhD, Director (DC)
(202) 687-0217
Michael.Johnson@@georgetown.edu
Research Building W416

Suman Ranjit, PhD, Assistant Director (DC)
Suman.Ranjit@georgetown.edu
Basic Science building 235B

Jan Blancato, PhD, Cytogenetics (DC)
(202) 444-1536
blancatj@georgetown.edu
LL184 Lombardi Building

Peter Johnson, Manager (DC)
(202) 687-1916 or 7-4296
pj42@georgetown.edu
Research Building W408

WenShan Tsao, Manager (NJ)
(201) 880-3495
wenshan.tsao@hmh-cdi.org
CDI Campus room A424

General Contact (reaches the whole team): MISRteam@georgetown.edu

Image of tumor cells stained for actin (green), HAI-2 (red), and DNA (blue)

Overview

The Microscopy & Imaging Shared Resource (MISR) provides access to a wide array of well-maintained instrumentation for light and electron microscopy, an array of image analysis platforms, and advise and expertise to assist investigators with their microscopy needs.   Imaging modalities supported range from conventional brightfield, wide-field florescence, laser scanning and spinning disk confocal, to more advanced techniques such as Förster or fluorescence resonance energy transfer (FRET), phasor-based fluorescence lifetime imaging (FLIM), fluorescence recovery after photobleaching (FRAP), second (and third) harmonic generation microscopy (SHIM), STED-based super-resolution microscopy, total internal reflection microscopy (TIRF), and multiphoton deep-tissue imaging.  The MISR supports the imaging of fixed samples and live cell and tissue preparations, with automated image acquisition over long time courses in various culture ware, on systems equipped with environmental chambers. 

The MISR staff provide advice on the optimal imaging approaches to achieving the desired research outcomes, assistance with experimental design, initial training and ongoing supervision with the equipment as needed, and help with image analysis and data interpretation. 

Location

  • Research Building W407, Georgetown University, 3970 Reservoir Rd NW, Washington, DC 20007
  • Preclinical Science LC-2, Georgetown University, 3900 Reservoir Rd NW, Washington, DC 20007
  • Preclinical Science LE-9, Georgetown University, 3900 Reservoir Rd NW, Washington, DC 20007
  • Center for Discovery & Innovation A424, 111 Ideation Way, Nutley, NJ 07110

MISR Intranet

Microscopy & Imaging Shared Resource is on LombardiNet where you can access the MISR intranet site for more information including descriptions of the equipment available, reagents, protocols and much more. A Georgetown University NetID and password are required.