Animal Model Zebrafish Core

Eric Glasgow, PhD
Telephone: (202) 687-7350
eg239@georgetown.edu

Green zebrafish swimming

Description

The role of the Zebrafish Shared Resource (ZSR) is to give investigators ready access to fish and fish embryos, and expertise in the use of the zebrafish model organism. Zebrafish are amenable to a wide range of experimental manipulations because zebrafish embryos are easily obtained in large numbers, they are externally fertilized, transparent, permeable to small molecules and drugs, and undergo rapid organogenesis. In addition, they have many of the same tissues and organ systems as humans. Moreover, due to their small size, large numbers of fish can be housed for relatively low cost, facilitating genetic studies and the generation of numerous transgenic and mutant fish lines. The resource director, who has over 15 years of experience working with zebrafish embryos, works closely with investigators to determine how zebrafish might be utilized to advance their cancer-related research. 

The award-winning documentary Zebrafish: Practically People, Transforming the Study of Disease highlights the way these fish are being used in labs as a biomedical research model.

External users: Science Exchange

Internal users: Click here for instrument scheduling

Services

  • Maintains a breeding colony of zebrafish including several wild-type strains, in addition to several mutant and transgenic lines that are highly useful for cancer-related research.
  • Provides consultation on potential uses of the zebrafish model to advance specific research projects and participates in the design of these experiments.
  • Performs zebrafish experiments and provides a range of services relating to the experimental use of this organism. These experiments include:
    • Microinjecting eggs with antisense Morpholino oligonucleotides (MO) to knock down specific genes on interest
    • Microinjecting eggs with RNA or cDNA to over-express wild-type, mutant and chimeric genes
    • Screening for toxicity/drugs
    • Generating transgenic zebrafish
    • Transplanting cells and xenotransplantation experiments
  • Provides expertise in the analyses of zebrafish phenotypes resulting from experimental manipulation.
  • Provides training at all levels in the use of zebrafish as a model system.

Fees

Service UnitRate
Fish Preparation for ExperimentHourly$30/hour
Training in Experimental TechniqueHourly$30/hour
Execution of Experiment Hourly$30/hour
Morphological and Data AnalysisHourly$60/hour
Stereoscopic PicturesHourly $60/hour
Pilot Morpholino Study*Per Exp.$1000/study
Chemical Screen EmbryosPer Embryo$20/50 embryos
Uninjected EmbryosPer Embryo $20/50 embryos
Single-Injected EmbryosPer Embryo $150/50 embryos
Double-Injected EmbryosPer Embryo $200/50 embryos
TUNEL AssayPer Assay$100
ImmunohistochemistryPer Assay $300
BrdUPer Assay $300
Acridine Orange AssayPer Assay $50
In Situ HybridizationPer Assay$50
Embryos: fixed and stagedPer embryo$50/50 embryos
Reagents 100% of cost
CRISPR mutantconfirmed mutant$1,250
CRISPR constructinjection ready$125
Genomic DNA extraction50 embryos$55
RNA extraction50 embryos$105
Protein extraction50 embryos$125

*Includes splice blocker and translation blocker injections along with dose response injections and analysis of resulting phenotype(s)

Acknowledgments
In all publications that include data derived or methods used from the Animal Models Shared Resource, please acknowledge our resource. The Animal Models Shared Resource is partially supported by NIH/NCI grant P30 CA051008.