Animal Models Zebrafish Shared Resource
Fees and Scheduling
Service | Unit | Rate |
---|---|---|
Fish Preparation for Experiment | Per experiment | $100 |
Training in Experimental Technique | Hourly | $60/hour |
Execution of Experiment | Hourly | $60/hour |
Morphological and Data Analysis | Hourly | $60/hour |
Stereoscopic Pictures | Hourly | $60/hour |
Pilot Morpholino Study* | Per experiment | $1000/study |
Chemical Screen Embryos | Per Embryo | $20/50 embryos |
Uninjected Embryos | Per Embryo | $20/50 embryos |
Single-Injected Embryos | Per Embryo | $150/50 embryos |
Double-Injected Embryos | Per Embryo | $200/50 embryos |
TUNEL Assay | Per Assay | $100 |
Immunohistochemistry | Per Assay | $300 |
BrdU | Per Assay | $300 |
Acridine Orange Assay | Per Assay | $50 |
In Situ Hybridization | Per Assay | $50 |
Embryos: fixed and staged | Per embryo | $50/50 embryos |
Reagents | – | 100% of cost |
CRISPR mutant | Confirmed mutant | $1,500 |
CRISPR construct | injection ready | $125 |
Genomic DNA extraction | 50 embryos | $55 |
RNA extraction | 50 embryos | $105 |
Protein extraction | 50 embryos | $125 |
Scheduling and Billing
External users: Contact the ZSR through the Science Exchange
Internal users: Click here for instrument scheduling
Georgetown/LCCC Consortium users: Contact the ZSR at zebrafishcore@gmail.edu to set up an initial consultation. Billing will be performed through Idea Elan after service is complete. In some instances, an initial payment may be required to cover cost of materials and reagents.
Operational Workflow
To ensure efficiency and optimal results, resource members interact closely with investigators. In order to utilize the Shared Resource, users will follow this basic operational workflow:
- An investigator directs an initial inquiry to zebrafishcore@gmail.com.
- An initial meeting is held during which the feasibility of the project and the overall research plan are discussed. The investigator is also notified of the service charges.
- Once deemed feasible, all pertinent information including laboratory personnel dedicated for the project and a grant/funding account for billing will be provided by the user.
- Investigator lab personnel undergo training.