Animal Models Zebrafish Shared Resource

Fees and Scheduling

*Includes splice blocker and translation blocker injections along with dose response injections and analysis of resulting phenotype(s)
Service UnitRate
Fish Preparation for ExperimentPer experiment$100
Training in Experimental TechniqueHourly$60/hour
Execution of Experiment Hourly$60/hour
Morphological and Data AnalysisHourly$60/hour
Stereoscopic PicturesHourly $60/hour
Pilot Morpholino Study*Per experiment$1000/study
Chemical Screen EmbryosPer Embryo$20/50 embryos
Uninjected EmbryosPer Embryo $20/50 embryos
Single-Injected EmbryosPer Embryo $150/50 embryos
Double-Injected EmbryosPer Embryo $200/50 embryos
TUNEL AssayPer Assay$100
ImmunohistochemistryPer Assay $300
BrdUPer Assay $300
Acridine Orange AssayPer Assay $50
In Situ HybridizationPer Assay$50
Embryos: fixed and stagedPer embryo$50/50 embryos
Reagents– 100% of cost
CRISPR mutantConfirmed mutant$1,500
CRISPR constructinjection ready$125
Genomic DNA extraction50 embryos$55
RNA extraction50 embryos$105
Protein extraction50 embryos$125

Scheduling and Billing

External users: Contact the ZSR through the Science Exchange

Internal users: Click here for instrument scheduling

Georgetown/LCCC Consortium users: Contact the ZSR at zebrafishcore@gmail.edu to set up an initial consultation.  Billing will be performed through Idea Elan after service is complete.  In some instances, an initial payment may be required to cover cost of materials and reagents.

Operational Workflow

To ensure efficiency and optimal results, resource members interact closely with investigators. In order to utilize the Shared Resource, users will follow this basic operational workflow:

  1. An investigator directs an initial inquiry to zebrafishcore@gmail.com.
  2. An initial meeting is held during which the feasibility of the project and the overall research plan are discussed. The investigator is also notified of the service charges.
  3. Once deemed feasible, all pertinent information including laboratory personnel dedicated for the project and a grant/funding account for billing will be provided by the user.
  4. Investigator lab personnel undergo training.