Zebrafish Equipment

The ZSR is equipped with standard instrumentation, such as a centrifuge, a water bath, agarose gel apparatus, power supplies, a thermo-cycler for PCR, two refrigerators, -20C freezer, two incubators, scales, and a transilluminator.

For microinjections, there is a picospritzer, micromanipulator and stereoscope.

For imaging, the ZSR is equipped with a Cannon Digital Color Camera to take color pictures on the Nikon SMZ-1500 EPI-Fluorescence and Transmitted Light Stereoscope System as part of the Microscopy and Imaging Shared Resource.

For higher resolution imaging, the ZSR recently purchased EC Plan NEOFLUAR 10x and 20x long working distance objectives, DIC prisms and AxioVision extended focus, z-stack, and panorama software upgrades for an AxioCam camera, in order to facilitate photography of zebrafish embryos on a shared use Zeiss Axioplan 2 microscope.

The ZSR is equipped with an Aquatic Habitats custom multi-rack recirculation system with the capacity to house up to 15,000 fish. In addition, an Aquatic Habitats stand-alone multi-rack system with the capacity to hold 1,600 fish is used for quarantine.

Fish Lines Available

Wild Type:

EK

Mutant:

med12y81

tp53zdf1

ptenbhu1435

apchu745

rag2e450fs; roya9/a9; mitfaw2/w2

c-myb t25127

prkdcD3612fs

Pigmentation Mutants:

cx41.8t1/t1 (leopard)

roya9/a9

mitfab692/b692 (nacre)

mitfaw2/w2

roya9/a9); mitfab692/b692 (casperina)roya9/a9; mitfaw2/w2 (casper)

albb4/b4 (albino)

Transgenic:

Tg(fli1a:nEGFP)y7

Tg(flh:EGFP)c161

Tg(flh:mCherry)

Tg(flh:mCherry-CAAX)

Tg(cmlc2:Venus-hGeminin)pd58

Tg(cmlc2:mCherry-zcdtl)pd57

Tg(mpeg1:GFP)

Tg(mpeg1:mCherry

Tg(-1.8gsc:GFP)ml1

Tg(elavl3:EGFP)zf8

Tg(isl1:GFP)rw0

Tg(mylz2:RFP)gz9

Tg(mylz2:YFP)gz10

Tg(mylz2:GFP)gz8

Tg(krt4:GFP;mylz2:RFP)gz13

Tg(oxt:EGFP)gu1

Tg(oxt:EGFP)gu9

Tg(oxt:EGFP)gu10

Tg(oxt:EGFP)gu15