Microscopy and Imaging Shared Resource (MISR)
Fees and Scheduling
Investigators, once they have completed the mandatory initial training for a given instrument, are then free to book time on the instrument through the Idea Elan portal. Investigators should contact Michael Johnson (misr@georgetown.edu) to schedule initial training.
Users are billed for the time that they actually spend using the instruments. Training and extended assistance is also billed on an hourly basis, although there is no charge for short (< 15 minutes) sessions with MISR staff to answer questions, help with trouble shooting etc. There is no charge for initial consultations, project planning, and assistance with experimental design and imaging strategy.
| Instrument/Service/Supplies (DC campus) | Rate |
|---|---|
| Leica SP8AOBS++ High speed live cell confocal, FREG, FRAP, MultiSpectral, FRET | $20/hour |
| Olympus FVMPE-RS with Diver detector Multiphoton, FLIM, Multiharmonic imaging, Live-cell and deep tissue imaging. | $20/hour (Investigator-operated) $80/hour (MISR-operated) |
| Abberior STEDYCON STED – super resolution, FLIM, live-cell imaging | $20/hour |
| Nikon E600 / Nikon SMZ1500 / Olympus IX-71 Epifluorescence, Stereo and Multispectral | $20/hour |
| Zeiss LSM 800 LSM confocal, long time-course live cell imaging, cell fate tracking | $20/hour (<8 hours) $16/hour (8-24 hours) $12/hour (24-100 hours) $8/hour (>100 hours) $5/hour (>200 hours) |
| Microscopy Assistance Training, sample prep, imaging/analysis, on-line supervision | $24/hour |
| Image Analysis Workstation Metamorph, Volocity, Image J-AIM | $8/hour |
| Hitachi H7600 TEM Transmission electron microscopy | $20/hour |
| Reichert Ultracut E Ultra-microtome TEM sample prep | $20/hour |
| Supplies Glass Bottom Dishes 12-well Glass Bottom MatTek Plates Antibody aliquots DNA construct aliquots | price $2/each $26/each $10/each $10/each |
| Instrument/Service/Supplies (NJ campus) | Rate |
| Nikon Ti2 A1R Confocal Confocal Microscopy | $20/hour |
| Nikon Ti2 w/Hamatsu Digital Camera Epi-Fluorescence Microscopy | $20/hour |
| Leica Laser Capture Dissection (LDM6 & LDM7) Laser Capture Microscopy | $20/hour |
| Image Processing and Analysis HP Desktop w/NIS-Advanced Research | $10/hour |
Operational Workflow
For all general inquires, feel free to use the Team email: MISRteam@georgetown.edu
For specific inquiries:
Experimental design, first use discussion: Michael Johnson @ johnsom@georgetown.edu
- Equipment signup and technical questions: Peter Johnson (DC) @ pj42@georgetown.edu, WenShan (NJ) @ wenshan.tsao@hmh-cdi.org )
- Initial training, troubleshooting: Peter Johnson (DC) @ pj42@georgetown.edu; Suman Ranjit (DC) Suman.Ranjit@georgetown.edu; WenShan (NJ) @ wenshan.tsao@hmh-cdi.org )
- CytogeneticsFISH methods: Jan Blancato @ blancatj@georgetown.edu
- Image analysis and live imaging: Peter Johnson (DC) @ pj42@georgetown.edu; Suman Ranjit (DC) Suman.Ranjit@georgetown.edu
- Molecular biology reagents – fluorescent protein markers, fusion constructs etc: Peter Johnson (DC) @ pj42@georgetown.edu
- Antibody aliquots: Peter Johnson (DC) @ pj42@georgetown.edu
- Tissue cultureware and other supplies: Peter Johnson (DC) @ pj42@georgetown.edu