Histopathology and Tissue Shared Resource

• Contact: Anju Duttargi ad1155@georgetown.edu.
• Tissue Collection — Fresh Frozen Tissues
  • Consent from Georgetown University Hospital (GUH) patients are obtained for the collection of excess tissues.
  • Fresh human tissues are collected, frozen, stored, and inventoried from various anatomic sites: Brain, Breast, Colon, Esophagus, Kidney, Liver, Lung, Pancreas, Stomach, Thyroid, Uterus.
  • Requests for frozen specimens must go through a specific approval process by the Biospecimen Use Committee. The review is based on scientific merit and statistical validity of the proposed project, amongst other factors.
• Tissue Collection — MGUH Archival Paraffin Embedded Tissues
  • Paraffin embedded human tissue blocks are available from the archives of the Pathology Department of the MedStar Georgetown University Hospital.
  • HTB conducts database searches of pathology records, as requested, for retrieval of human tissue blocks. Patient confidentiality is maintained in accordance with HIPAA regulations.
  • Sectioning of archival blocks is conducted in the Histology Laboratory.
  • All protocols requiring human material must have IRB approval and must adhere to HIPAA guidelines. HTB maintains an annually renewable IRB approval allowing for LCCC investigators to use the archived patient material in a de-identified fashion.
  • Permission for tissue retrieval can be granted to investigators after a one-time short application process.
  • All investigators complete a HTB Repository Use Form {link} to utilize HTSR services.

Contact: Anju Duttargi (ad1155@georgetown.edu) or Elizabeth Jones (ej275@georgetown.edu).

Histology services include:

• The Histology Laboratory interacts closely with the Human Tissue Bank to provide unstained and stained tissue sections from the appropriately chosen paraffin blocks that fulfill the investigator’s research
• Tissue fixation and processing for paraffin and frozen tissue blocks
• Paraffin and frozen tissue microtomy
• Routine and non-routine histology stains
• Customized services consultation and advice for fixation and tissue preparation to help meet specific research goals.

Contact: For routine IHC, please contact Supti Sen (ss395@georgetown.edu) or Karma Cook (kc1502@georgetown.edu). For multiplex IHC, please contact Susree Modepalli (sm3650@georgetown.edu).

The laboratory provides the following services:

• Two DAKO Autostainers for high throughput immunohistochemical staining
• A Leica BOND autostainer for multiplex staining
• Protocol development using new antibodies from commercial vendors or custom antibodies.
• Optimization of staining intensity and specificity
• Assisting with interpretation
• Quantitative analysis of chromogenic staining with Aperio GT450 scanner and quantitative fluorescent immunostaining with Vectra 3

Contact: Susree Modepalli (sm3650@georgetown.edu) or Dylan Kutz (dk1120@georgetown.edu).

The laboratory is equipped with:

• Dual headed microscopes

• Aperio GT450: Automated, high capacity digital slide scanner (brightfield only)
• Phenocycler Fusion System
  • Automated fluorescent and high-resolution ROI imaging
  • InForm: Automated image analysis software

• Dr. Galam Khan is available to review slides with investigators on an individual basis. Appointments can be made via email, please contact Anju Duttargi (ad1155@georgetown.edu) to schedule a consultation.