Proteomics Shared Resource
Junfeng Ma, PhD, Director
Office: GD-11 Preclinical Science Building,
Georgetown University Medical Center,
3970 Reservoir Rd NW,
Washington, DC 20057
The proteomics section is equipped with TripleTOF 6600 and QTRAP 6500 mass spectrometers (Sciex) which can be interfaced with nanoAcquity UPLC systems (Waters) or cHiPLC enabled 2D nanoLC system (Sciex). In addition, the shared resource houses an Agilent 1100 nanoflow HPLC interfaced with an LC Packings Probot, 2D gel electrophoresis system (BioRad) with an imaging unit for DIGE quantification (GE Healthcare), Acquity UPLC with fluorescent detector, and an ELISA ECL sector imager 2400 (Mesoscale).
The high speed, sensitivity, and accuracy of our mass spectrometers allow accurate qualitative and quantitative measurements. The most common experiments include descriptive proteomics studies of biological systems, studies of proteins and their modifications, and quantification of proteins including iTRAQ labeling, data independent quantification (SWATH), targeted quantification by using multiple reaction monitoring (MRM) or parallel reaction monitoring (PRM).