Image of protein chain

Proteomics Metabolomics

Equipment and Fees
Contact Information and Staff
Educational Resources

 

The proteomics section is equipped with TripleTOF 5600 and QTRAP 6500 mass spectrometers (Sciex) which can be interfaced with cHiPLC enabled 2D nanoLC system (Sciex) or nanoAcquity UPLC (Waters). In addition, the shared resource houses an Agilent 1100 nanoflow HPLC interfaced with an LC Packings Probot, 2D gel electrophoresis system (BioRad) with an imaging unit for DIGE quantification (GE Healthcare), Acquity UPLC with fluorescent detector, and an ELISA ECL sector imager 2400 (Mesoscale).

The high speed, sensitivity, and accuracy of our mass spectrometers allow accurate qualitative and quantitative measurements. The most common experiments include descriptive proteomics studies of biological systems, studies of proteins and their modifications, and quantification of proteins including iTRAQ labeling, LC-MS/MS-MRM, and targeted proteomics including data independent quantification strategies.

The metabolomics section houses QTOF Premier (Waters Corp.) online with an UPLC and Xevo G2 QTOF online with a UHPLC system for untargeted metabolomic profiling and drug metabolism studies. The data processing for metabolite biomarker studies is supported by the 'SIMCA-P' and the 'Random Forest software.' PMSR also manages a Xevo TQ-S triple quadrupole system which support quantitation and validation for small-molecule metabolites. PMSR staff includes a dedicated bio-informatician who works closely with the Biostatistics and Bioinformatics Shared Resource (BBSR).

The metabolomic component was recognized at the Waters Center of Innovation for metabolomics and has supported over 30 publications, invited talks and enabled successful funding of cancer-centric grant proposals. Cheema serves as a member of the "Metabolomics Research Group" of the ABRF (American Biomolecular Research Facilities). Written and electronic reports are provided for all samples, and the average turn-around time is two weeks.

Click here to access the Metabolomics website.

Radoslav Goldman, PhD, Director
Telephone: (202) 687-9868
rg26@georgetown.edu
LCCC, LL (S) Level, Room S183

Junfeng Ma, PhD, Research Scientist
Telephone: (202) 687-3802
jm3004@georgetown.edu
Preclinical Science Building GD9

Miloslav Sanda, PhD
Telephone: (202) 687-6278
ms2465@georgetown.edu
Preclinical Science Building GD9

Amrita Cheema, PhD, Co-director
Telephone: (202) 687-2756
akc27@georgetown.edu
Preclinical Science Building GD9

Albert J Fornace, Jr, MD, Co-director
Telephone: (202) 687-7843
af294@georgetown.edu
Research Building E504

 

Acknowledgements

In all publications that include data derived or methods used from the Proteomics & Metabolomics Shared Resource, please acknowledge our resource. The Proteomics & Metabolomics Shared Resource is partially supported by NIH/NCI grant P30 CA051008.