Tissue Culture

Shared Resource


The role of Tissue Culture Shared Resource (TCSR) is to assist the Cancer Center investigators and other members of the Georgetown University research community with all the tissue culture-related aspects of their research and to maintain the Lombardi cell line repository. To this end the Resource provides consultation, facilities and services to a diverse group of investigators involved in cancer research.

The services offered by the TCSR include the following:

  • Generates primary cell lines from normal and tumor tissues, using a novel Conditionally Reprogrammed Cell (CRC) technology.
  • Provides a B-cell transformation service to facilitate cancer genetics studies.
  • Provides quality control tests for cell lines including mycoplasma testing and genetic fingerprinting of cells.
  • Provides cell culture and banking service.
  • Provides a safe, secure cryo-storage facility for investigators to keep frozen stocks of their cancer cell lines.
  • Prepares, tests and supplies tissue culture reagents of the highest quality, that have been specifically tested for suitability in the relevant systems, as inexpensively as possible.
  • Equips, monitors, stocks and services shared tissue culture laboratories for the use of investigators who do not have facilities to perform tissue culture in their own laboratories or who need additional tissue culture space.
  • Maintains the Lombardi biorepository of cell lines.
  • Provides processing and banking of blood and other biofluids for Lombardi cancer patient biorepository.
  • Provides expert consultation and training regarding the use of tissue culture techniques in cancer research.

Contact Information

Director: Bassem Haddad, MD
Telephone: (202) 444-0759
Lombardi Building, LL S-111

Manager: Dionyssia Clagett, MS
Telephone: (202) 687-0240
Research Building, W314

New investigators to the Cancer Center as well as first time users should contact the Director or the Manager to become familiar with our facility, and to discuss any specific needs that they may have. More information is available on LombardiNet.


In all publications that include data derived or methods used from the Tissue Culture Shared Resource, please acknowledge our resource. The Tissue Culture Shared Resource is partially supported by NIH/NCI grant P30 CA051008.