Proteomics & Metabolomics Shared Resource
Co-Directors of Metabolomics
Amrita Cheema, PhD
Telephone: (202) 687-2756
Preclinical Science Building GD9
Albert J Fornace, Jr, MD
Telephone: (202) 687-7843
Research Building E504
Co-director of Proteomics
Radoslav Goldman, PhD
Telephone: (202) 687-9868
LCCC, LL (S) Level, Room S183
Facility Manager: Lihua Zhang, MS
Telephone: (202) 687-3802
Preclinical Science Building GD9
Preclinical Science Building GD9 & GD11
3900 Reservoir Rd NW
Washington, DC 20057
The PMSR was established in January 2006 and has grown dramatically, both in resources and use. Lombardi has invested over $1 million in equipment alone for the PMSR; as a result, the facility consistently provides high quality data to many investigators from 5 programs within Lombardi. This centralized shared resource is available to researchers for routine and specialized proteomic and metabolomic applications. The aim of PMSR is to provide an 'in house' resource, rich in technical expertise that fosters the free flow of experimental knowledge and collaborations across the Lombardi Comprehensive Cancer Center (Lombardi).
The PMSR offers an array of proteomics- and metabolomics-based services. It is equipped with two-dimensional (2D) gel electrophoresis apparatus including DIGE imager and spot picker (GE Healthcare), 4800 MALDI TOF-TOF instrument (ABI), and a QSTAR Elite Hybrid LC MS/MS system (ABI) to support the proteomic workflow. Each instrument has the latest integrated software and database search engines (e.g., Protein Pilot, GPS explorer) for protein identification and data processing. In addition, PMSR houses QTOF Premier (Waters Corp.) online with an UPLC and Xevo G2 QTOF online with a UHPLC system for untargeted metabolomic profiling and drug metabolism studies. The data processing for metabolite biomarker studies is supported by the 'SIMCA-P' and the 'Random Forest software'. PMSR also manages a Xevo TQ-S triple quadrupole system and a 4000 QTrap, which support quantitation and validation for small-molecule metabolites, and allow targeted proteomic analysis. PMSR staff includes a dedicated bio-informatician who works closely with the Biostatistics and Bioinformatics Shared Resource (BBSR), as well as with the Georgetown University-based Protein Information Resource (PIR).
The most common proteomic experiments include identification of proteins contained in bands/spots isolated from electrophoretic gels, as well as comparative proteomics using shotgun and stable isotope labeling strategies. The high speed, sensitivity, and accuracy of our mass spectrometry experiments allow accurate qualitative and quantitative proteomics. Â The metabolomic component was recognized at the Waters Center of Innovation for metabolomics and has supported over 30 publications, invited talks and enabled successful funding of cancer-centric grant proposals. Cheema serves as a member of the "Metabolomics Research Group" of the ABRF (American Biomolecular Research Facilities). Written and electronic reports are provided for all samples, and the average turn-around time is two weeks. The PMSR is able to carry out experiments that detect and characterize protein modifications such as phosphorylation and acetylation. These experiments are challenging and require a more intensive effort. However, the combination of the complementary mass spectrometric methods available in PMSR, staff expertise and experience, and the ability to work closely with investigators, have allowed the successful completion of a number of these difficult projects.
AcknowledgementsIn all publications that include data derived or methods used from the Proteomics & Metabolomics Shared Resource, please acknowledge our resource. The Proteomics & Metabolomics Shared Resource is partially supported by NIH/NCI grant P30 CA051008.