Proteomics & Metabolomics

Shared Resource


Proteomics & Metabolomics Shared Resources (PMSR) was established in January 2006 and has grown dramatically, both in resources and use. Lombardi Comprehensive Cancer Center (LCCC) has invested over $1 million in equipment alone for the PMSR; as a result, the facility consistently provides high quality data to many investigators from 4 programs within LCCC. This centralized shared resource is available to researchers for routine and specialized proteomic and metabolomic applications. The aim of PMSR is to provide an 'in house' resource, rich in technical expertise that fosters the free flow of experimental knowledge and collaborations across the Georgetown University.

PMSR offers an array of proteomics and metabolomics services. The proteomics section is equipped with TripleTOF 5600 and QTRAP 6500 mass spectrometers (Sciex) which can be interfaced with cHiPLC enabled 2D nanoLC system (Sciex) or nanoAcquity UPLC (Waters). In addition, the shared resource houses an Agilent 1100 nanoflow HPLC interfaced with an LC Packings Probot, 2D gel electrophoresis system (BioRad) with an imaging unit for DIGE quantification (GE Healthcare), Acquity UPLC with fluorescent detector, and an ELISA ECL sector imager 2400 (Mesoscale). The metabolomics section houses QTOF Premier (Waters Corp.) online with an UPLC and Xevo G2 QTOF online with a UHPLC system for untargeted metabolomic profiling and drug metabolism studies. The data processing for metabolite biomarker studies is supported by the 'SIMCA-P' and the 'Random Forest software'. PMSR also manages a Xevo TQ-S triple quadrupole system which support quantitation and validation for small-molecule metabolites. PMSR staff includes a dedicated bio-informatician who works closely with the Biostatistics and Bioinformatics Shared Resource (BBSR), as well as with the Georgetown University-based Protein Information Resource (PIR).

The high speed, sensitivity, and accuracy of our mass spectrometers allow accurate qualitative and quantitative measurements. The most common experiments include descriptive proteomics studies of biological systems, studies of proteins and their modifications, and quantification of proteins including iTRAQ labeling, LC-MS/MS-MRM, and targeted proteomics including data independent quantification strategies. The metabolomic component was recognized at the Waters Center of Innovation for metabolomics and has supported over 30 publications, invited talks and enabled successful funding of cancer-centric grant proposals. Cheema serves as a member of the "Metabolomics Research Group" of the ABRF (American Biomolecular Research Facilities). Written and electronic reports are provided for all samples, and the average turn-around time is two weeks. The combination of the complementary mass spectrometric methods available in PMSR, staff expertise and experience, and the ability to work closely with investigators, have allowed the successful completion of a number of challenging projects.

Contact Information

Co-Directors of Metabolomics
Amrita Cheema, PhD
Telephone: (202) 687-2756
Preclinical Science Building GD9

Albert J Fornace, Jr, MD
Telephone: (202) 687-7843
Research Building E504

Director of Proteomics
Radoslav Goldman, PhD
Telephone: (202) 687-9868
LCCC, LL (S) Level, Room S183

Research Scientists, Proteomics
Junfeng Ma, PhD
Telephone: (202) 687-3802
Preclinical Science Building GD9

Miloslav Sanda, PhD
Telephone: (202) 687-6278
Preclinical Science Building GD9


In all publications that include data derived or methods used from the Proteomics & Metabolomics Shared Resource, please acknowledge our resource. The Proteomics & Metabolomics Shared Resource is partially supported by NIH/NCI grant P30 CA051008.