Microscopy & Imaging Shared Resource

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Contact

Director: Michael Johnson, PhD
(202) 687-0217
johnsom@georgetown.edu
Research Building E401

Director of Dynamic Imaging: Position Open
Michael Johnson, PhD
johnsom@georgetown.edu
Research Building E401

Cytogenetics: Jan Blancato, PhD
(202) 444-1536
blancatj@georgetown.edu
LL84 Lombardi Building

Manager: Peter Johnson
(202) 687-1916 or 7-4296
pj42@georgetown.edu
Research Building W408

Consultant: Susette C. Mueller, PhD
(301) 651-6869
muellers@georgetown.edu

Address:
Research Building Suite W407
Georgetown University
3970 Reservoir Rd NW
Washington, DC 20057

For specific inquiries:

• Experimental design, first use discussion: johnsom@georgetown.edu

• Collaboration: johnsom@georgetown.edu

• Equipment signup and technical questions: pj42@georgetown.edu

• Image analysis and live imaging: pj42@georgetown.edu

Description

The Microscopy & Imaging Shared Resource (MISR) provides instrumentation and expertise for light microscopy. Users incorporate a variety of cell biological techniques into their studies including total internal reflection microscopy (TIRF), laser scanning and spinning disk confocal, and automated image acquisition for screening of multiwell samples. Applications include fluorescence resonance energy transmission (FRET), fluorescence lifetime intensity microscopy (FLIM) for FRET measurements, fluorescence recovery after photobleaching (FRAP), and other live imaging applications; multispectral emission imaging, and epifluorescence and brightfield time-lapse microscopy, microinjection, immuno-light microscopy and image analysis to study tumor cell biology.

Acknowledgements

In all publications that include data derived or methods used from the Microscopy & Imaging Shared Resource, please acknowledge our resource. The Microscopy & Imaging Shared Resource is partially supported by NIH/NCI grant P30-CA051008.