Microscopy and Imaging Shared Resource

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Equipment (List by Microscope)

Cytogenetics Services

Equipment (List by Imaging Method)

Tissue (in vivo or ex vivo)

Fluorescence

• Nikon SMZ-1500 EPI-Fluorescence Digital Stereoscope System Surface Imaging.
• Zeiss LSM510/META/NLO with Chameleon XR Ti-Sapphire laser Multiphoton deep tissue cellular imaging

Cell

Bright field

• Color camera for brightfield 24 bit color representation
• Inverted
  • Olympus IX71 Inverted Digital Microscope System (10X-40X)
• Upright
  • Nikon E600 Multispectral Epi-Fluorescence Microscope System (10X-100X)
  • Olympus AH2 Vanox Digital Microscope System (10X-40X)

Phase contrast

• Inverted
  • Olympus IX71 Inverted Digital Microscope System (10X-40X)
  • Nikon TE300 Spinning Disk Time Lapse Microscope System (60X-100X)
  • Olympus Fluoview-FV300 Laser Scanning Confocal System (10X, 40X)
  • Zeiss IM-35 Inverted Microscope System (6.3X-40X) (microinjection, bright field)
• Upright
  • Nikon E600 Multispectral Epi-Fluorescence Microscope System (10X-100X)

 

Differential interference contrast (Nomarski)

• Inverted
  • Olympus Fluoview-FV300 Laser Scanning Confocal System (20X-100X)
  • Nikon TE300 Spinning Disk Time Lapse Microscope System (60X-100X)
  • Zeiss 510LSM/ META/ NLO live imaging, multiphoton microscope (10X-60X)

 

Fluorescence

• Widefield (Epi-fluorescence)
• Inverted
  • Olympus IX71 Inverted Digital Microscope System (10X-40X)
  • Nikon SMZ-1500 EPI-Fluorescence Digital Stereoscope System (0.5X-1.0X)
• Upright
  • Olympus AH2 Vanox Digital Microscope System (10X-40X)
  • Nikon E600 Multispectral Epi-Fluorescence Microscope System (10X-100X)

 

Laser scanning confocal

• Inverted
  • Olympus Fluoview-FV300 Laser Scanning Confocal System (10X-100X)
  • Zeiss LSM510/META/NLO

 

Spinning disk confocal

• Inverted
  • Nikon TE300 Spinning Disk Time Lapse Microscope System (60X-100X)
  • Olympus BX61 DSU Fluorescent scope (Histopathology and Tissue SR)
    (this scope is for very bright samples such as tissue slices)

 

Interference reflection (IRM)

• Olympus IX81 Total Internal Reflection Microscope (10x, 60X, 150X)
• Olympus Fluoview-FV300 Laser Scanning Confocal System (10X-100X)
• Zeiss 510LSM/ META/ NLO live imaging, multiphoton microscope (10X-60X)

 

Total internal reflection microscopy (TIRF)

• Olympus IX81 Total Internal Reflection Microscope (60X, 150X)

 

Multispectral emission imaging

• Zeiss 510LSM/ META/ NLO live imaging, multiphoton microscope (10X-60X)
• Nikon E600 Multispectral Epi-Fluorescence Microscope System (10X-100X)
• http://www.cri-inc.com/products/nuance.asp

 

Fluorescence resonance energy transfer (FRET)

• Zeiss 510LSM/ META/ NLO live imaging, multiphoton microscope (10X-60X)
• Olympus Fluoview-FV300 Laser Scanning Confocal System (10X-100X)
• Olympus IX81 TIRF

 

Fluorescence lifetime intensity (FLIM)

• Zeiss 510LSM/ META/ NLO live imaging, multiphoton microscope (10X-60X) with Becker-Hickl FLIM

 

Fluorescence recovery after photobleaching (FRAP and FLIP)

• Zeiss 510LSM/ META/ NLO live imaging, multiphoton microscope (10X-60X)
• Olympus Fluoview-FV300 Laser Scanning Confocal System (10X-100X)

 

Fluorescence loss in photobleaching (FLIP)

• Zeiss 510LSM/ META/ NLO live imaging, multiphoton microscope (10X-60X)

 

Multiwell and Time Lapse Automated Image Acquisition

• Nikon TE300 Spinning Disk Time Lapse Microscope System (60X-100X)
• Molecular Devices ImageXpressMICRO (4X-40X)

 

Time Lapse

• Zeiss IM-35 Inverted Microscope System (6.3X-40X) (microinjection, bright field)
• Nikon TE300 Spinning Disk Time Lapse Microscope System (60X-100X)
• Zeiss 510LSM/ META/ NLO live imaging, multiphoton microscope (10X-60X)
• Olympus Fluoview-FV300 Laser Scanning Confocal System (10X-100X)
• Nikon SMZ-1500 EPI-Fluorescence Digital Stereoscope System (room temp)