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Zeiss LSM510/META/NLO Live Imaging Multiphoton Microscope

Zeiss LSM510Zeiss LSM510 Microscope


The Zeiss 510LSM/ META/ NLO live imaging, multiphoton microscope: The META component allows for spectral emission imaging to facilitate complete separation of fluorescent emissions with Lambda mode acquisition and Channel mode acquisition facilitates multi-fluorophore imaging in either single track (up to three fluorophores) or multi-track. This instrument will also be used for in vivo multiphoton imaging for high resolution, tissue imaging. Planned acquisition of single photon detection for fluorescence lifetime imaging (FLIM) to complement the existing fluorescence resonance energy transfer (FRET) capabilities.

Lasers:

  • Argon 458nm/488 nm/ 514 nm

  • HeNe 543 nm

  • HeNe 633 nm

  • Coherent Chameleon XR Ti:Sapphire laser (705nm-980 nm)

Software:

  • Zeiss 510 META with Physiology Software ver. 3.5 and Multiple Time Series Software ver. 3.5

Objectives:

  • 10X/ 0.3 N.A.

  • 40X/ 1.3 N.A. OIL

  • 63X/ 1.4 N.A. OIL

  • 25X/ 0.8 N.A. W long working distance 0.49 beyond coverslip

  • 40X/ 1.2 N.A. W corr for high resolution tissue imaging

Acknowledgments:
PLEASE REMEMBER: For funding purposes, it is important to acknowledge the Microscopy & Imaging Shared Resource Facility in all publications that include data derived from the Facility. For Zeiss LSM510 microscope, this should include the following statement:

"This work was supported in part by the Lombardi Comprehensive Cancer Center Microscopy and Imaging Shared Resource funded by U.S. Public Health Service Grant 2P30-CA-51008, 1S10 RR15768-01, 1 S10 RR019291-01A2, and the Carey Lackman Slease Fund."

If you have comments or suggestions, email Michael Johnson, director of MISR at Johnsom@georgetown.edu.