Microscopy and Imaging Shared Resource

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Olympus IX71 Inverted Epi-Fluorescent Microscope

 

For routine viewing of tissue culture cells and imaging in brightfield or fluorescence with color CCD digital image acquisition. Phase contrast and DAPI, FITC, Cy3/ Texas Red.

Objectives:

• UPlanFL, 10X, N.A. 0.30, Ph1

• LCPlanFL, 20X, N.A. 0.40, Ph1

• LCPlanFL, 40X, N.A. 0.60, Ph2

Filter Cubes:

• DAPI/Hoechst: (excitation=330-380nm, dichroic=400nm, emission=420nm)

• FITC: (excitation=480nm, dichroic=505nm, emission=535nm)

• TRITC: (excitation=540nm, dichroic=565nm, emission=605nm)

Camera:

• Olympus DP70 Color / Black and White camera

Computer & Imaging Software:

• Olympus DP Capture Software

• Pentium III running Windows XP. 768 MB RAM, 40GB Hard Drive, Zip Drive, writable CD.

Acknowledgments:

PLEASE REMEMBER: For funding purposes, it is important to acknowledge the Microscopy & Imaging Shared Resource Facility in all publications that include data derived from the Facility.

"This work was supported in part by the Lombardi Comprehensive Cancer Center Microscopy and Imaging Shared Resource funded by U.S. Public Health Service Grant 2P30-CA-51008, 1S10 RR15768-01, 1 S10 RR019291-01A2, and the Carey Lackman Slease Fund."

If you have comments or suggestions, email Michael Johnson, director of MISR at Johnsom@georgetown.edu.