Microscopy and Imaging Shared Resource

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Nikon SMZ-1500 EPI-Fluorescence and Transmitted Light Stereoscope System

Objectives:

• Plan Apo, 0.5X (zoom range from 0.375X to 5.6X)

• Plan Apo, 1.0X (zoom range from 0.75X to 11.25X)

• Plan Apo, 1.6X (zoom range from 1.6X to 18X)

Filter Cubes:

• GFP: (excitationn= 480/40nm, dichroic= 505nm, emissionn= 535/50nm)

• DsRed: (excitation=545nm, dichroic=570nm, emission=620nm)

• Q-dot (excitation=460nm, dichroic=475nm, emission=655nm)

Camera:

• Hamamatsu Orca-100, 12-bit cooled CCD digital camera, 1392 x 1040 pixel array, 6.45 x 6.45um pixel size

Computer & Imaging Software:

• 1.0 GHz Pentium III, Windows 2000, CD/USB1 data transfer

• MetaMorph image aquisition and analysis software (version 6.3r7)

• Motorized z-stage for 3D, live cell imaging

• Even (Flat) Brightfield Illuminator

Acknowledgments:
PLEASE REMEMBER: For funding purposes, it is important to acknowledge the Microscopy & Imaging Shared Resource Facility in all publications that include data derived from the Facility. For Nikon SMZ 1500 Stereoscope microscope, this should include the following statement:

"This work was supported in part by the Lombardi Comprehensive Cancer Center Microscopy and Imaging Shared Resource funded by U.S. Public Health Service Grant 2P30-CA-51008, 1S10 RR15768-01, 1 S10 RR019291-01A2, and the Carey Lackman Slease Fund."

If you have comments or suggestions, email Michael Johnson, director of MISR at Johnsom@georgetown.edu