Microscopy and Imaging Shared Resource

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Nikon E600 Upright Epi-Fluorescence Microscope System with Phase Contrast

Nikon E600Nikon E600 Microscope


Objectives:

  • Plan Fluor, 10X, N.A. 0.30, WD= 16.0mm

  • Plan Fluor, 20X, N.A. 0.50, WD= 2.1mm

  • Plan Apo, 40X, N.A. 0.75, WD= 0.14mm

  • Plan Apo, 60X Oil, N.A. 1.40, WD= 0.21mm

Filter Cubes:

  • DAPI/Hoechst: (EX=330-380nm, DM=400nm, EM=420nm)

  • FITC: (EX=480nm, DM=505nm, EM=535nm)

  • TRITC: (EX=540nm, DM=565nm, EM=605nm)

  • CY5: (EX=620nm, DM=660nm, EM=700nm)

Camera:

  • CRi Nuance Multispectral Imaging System: Camera uses a liquid crystal optical tuning element to capture electronic image cubes across specifiable wavelengths, this allows spectral classification and unmixing of overlapping signals including unmixing of autofluorescence from dye signals, resulting in high definition/low background images. This system also operates with conventional bright field stains.

  • NOTE: Reference standards of each stain color plus a non-stained native standard for each experimental protocol are required for publishable results. Please consult with Dr. Susette Mueller (muellers@georgetown.edu) or Peter Johnson (pj42@georgetown.edu) to help design the experimental protocol.

Computer & Imaging Software:

  • 3.0GHz Intel dual core, Windows XP. CD/DVD or USB2 data transfer.

  • Nuance v2.4 Multispectral Imaging software, Cambridge Research & Instrumentation, Inc. (CRi)

Acknowledgments:
PLEASE REMEMBER: For funding purposes, it is important to acknowledge the Microscopy & Imaging Shared Resource Facility in all publications that include data derived from the Facility. For Nikon E600 Epi-Fluorescent microscope, this should include the following statement:

"This work was supported in part by the Lombardi Comprehensive Cancer Center Microscopy and Imaging Shared Resource funded by U.S. Public Health Service Grant 2P30-CA-51008, 1S10 RR15768-01, 1 S10 RR019291-01A2, and the Carey Lackman Slease Fund."

If you have comments or suggestions, email Michael Johnson, director of MISR at Johnsom@georgetown.edu.