Genomics and Epigenomics Shared Resource

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User-Operated Instruments

The following are user-operated instruments. GESR provides oversight, technical support, maintenance, calibration and training. For more information, contact Md Islam or Xiaojun Zou.

ABI Sequence Analyzer 7900HT. This instrument can be used for real-time polymerase chain reaction (Real-Time PCR) experiments.

GenePix 4000A Scanner. This instrument is connected to a computer with GenePix Pro software. It can be used for analysis of glass slide arrays in transcriptomics and proteomics studies.

Tecan Ultra Microplate Reader. This plate reader is capable of reading steady state fluorescence, time-resolved fluorescence, fluorescence polarization, and luminescence. This instrument is useful for screening lead drug compounds with high sensitivity. The Tecan Ultra has a flexible plate reading capacity ranging from six well plates to 386 well plates. The sensitivity for fluorescence (fluorescein) is 1 pM and for time resolved fluorescence (europium) is 3 amols. Precision for fluorescence polarization assays is 3 mP. The instrument is controlled by a computer that can also be used for data analysis.

Alpha Innotech FluorChem-8900. This instrument is equipped with a 12-bit peltier cooled CCD detection camera with filters with wavelengths of 595, 500, 460, and 630. It has light sources for transillumination and epiillumination of both visible and fluorescent samples. Images are captured as digital image files and are stored on the attached computer. The images can also be quantified using the software on the attached computer. Images can be printed using the attached thermal printer.

Beckman DU-640 Spectrophotometer. This instrument can be used to measure the UV or visible optical density of samples.

Wallac 1420 VICTOR2 96-Well Plate Reader. This instrument is capable of measuring flash or glow luminescence, fluorescence, high-sensitivity time-resolved fluorescence, flash absorbance, and photometry. It is connected to a PC computer for data collection and analysis.

Berthold V9. This instrument is a luminometer.

Amaxa Nucleofector. This instrument can be used for primary and culture cell transfections.

NanoDrop ND-1000 Spectrophotometer. This instrument is a full-spectrum (220-750nm) spectrophotometer that is used to measure samples as small as 1ul with high accuracy and reproducibility. It also has the capability to measure highly concentrated samples without dilution. The applications of NanoDrop ND-1000 spectrophotometer include for measuring nucleic acid concentration and quality of nucleic acid samples up to 3700 ng/ul (dsDNA) without dilution, fluorescent dye labeling density of nucleic acid microarray samples, and purified protein analysis (A280) up to 100 mg/ml (BSA).

Bio-Tek ELx808 Absorbance Microplate Reader. This instrument accepts 96-well plates; Absorbance: 0 to 4.0 O.D.; Wavelength: 340 to 900nm; Accuracy: <1% at 2.5 O.D., <2% at 3.5 O.D.

LI-COR Odyssey Infrared Imager. This instrument can been used for many applications, including Western blot analysis, EMSA, protein arrays, In-Cell Western™ Assays, On-Cell Westerns, in vivo imaging, Coomassie gel documentation, DNA gel documentation and tissue section analysis.

 

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