Genomics and Epigenomics Shared Resource

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Gene Expression Profiling

GESR provides gene expression profiling service through the following instruments:

Affymetrix Microarray System

  • Affymetrix GeneChip analysis system with fluidics station, hybridization oven, GeneChip scanner, and computer workstations for Affymetrix data acquisition, storage, and analysis of gene expression. Use the following link to complete online sample submission form for Affymetrix service: http://rnaseq.georgetown.edu/gesrweb/submission_affymetrix.php

Agilent Microarray System

  • Agilent system that includes a hybridization oven, Agilent DNA microarray scanner (G2565AA), and Feature Extraction software for analysis of gene expression. Use the following link to complete online sample submission form for Agilent service: http://rnaseq.georgetown.edu/gesrweb/submission_agilent.php

 

Illumina Microarray System

  • Illumina Human HT-12 v4 Expression Bead Chip Full Service on the HiScanSQ (sample preparation, labeling, hybridization, scanning, and data analysis).    

 

ABI TaqMan Gene Expression Assays

  • Using TaqMan Gene Expression Assays in Relative Quantitation mode on the ABI 7900HT Fast RT-PCR System enables the screening of a large number of samples using highly accurate and specific chemistry.

 

For information on Affymetrix assays, contact Xiaojun Zou, for Agilent, Illumina and ABI assays, contact David Goerlitz. For gene expression data analysis, contact James Li or Habtom Ressom.

 

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