Genomics and Epigenomics Shared Resource

GESR Home | Services | Fees | Publications | Schedule

Fees

 

Service Fee for LCCC Members
 
Gene Expression Profiling 
Affymetrix  
Microarray chip hybridization, staining, washing, and scanning (Partial service) $140/sample (chip purchased separately)
3' Gene Expression Analysis Full Service (sample preparation, labeling, hybridization, scanning, and basic data analysis*)  $325/sample (chip purchased separately)
Arrays including:  GeneChip Human Genome U133 Plus 2.0,  GeneChipMouse Genome 430A2.0, and GeneChip Rat Genome 230 2.0
Whole-Transcript Expression Analysis Full Service (sample preparation, labeling, hybridization, scanning, and basic data analysis*) $365/sample (chip purchased separately)
Arrays including Human Exon 1.0 ST Array & Gene1.0 Array for Human, Mouse and Rat.
Agilent   
Agilent array scan (hybridization, scanning, and basic data analysis*) $100/array (arrays purchased separately)
Agilent Gene Expression Full Service (sample preparation, labeling, hybridization, scanning, and basic data analysis*)

Arrays include 8x60K or 4x44K Human Gene Expression Microarrays
One-color: $200/array (arrays purchased separately) Two-color: $300/array (arrays purchased separately)
ABI  
ABI TaqMan Gene Expression assays $1.5/gene/sample + $60/plate
Illumina  
Illumina Human HT-12 v4 Expression BeadChip Full Service (sample preparation, labeling, hybridization, scanning, and basic data analysis*) $125/sample (includes cost of the array)
Illumina Human Whole-Genome DASL Assay for degraded RNA (FFPE): HT-12 v4 Expression BeadChip Full Service (sample preparation, labeling, hybridization, scanning, and basic data analysis*) $185/sample (includes cost of the array)
Illumina Mouse WG-6 v2.0 Expression BeadChip Full Service (sample preparation, labeling, hybridization, scanning, and basic data analysis*) $230/sample (includes cost of the array)
   
Next Generation Sequencing
Human Exome Sequencing Full Service (DNA QC, whole-exome capture using the Agilent SureSelect kit v4 (54Mb), library prep, indexing and 100 bp paired-end reads on the Illumina HiSeq2000/2500 (50X coverage). Raw FASTQ seq provided along with standard bioinformatics including reference genome mapping and variant calls. $1282/sample
Human RNA-Seq Full Service (RNA QC (Agilent Bioanalyzer), library preparation (Illumina TruSeq), indexing and 100 bp singleton reads on the Illumina HiSeq2000/2500 (20M reads/sample). Raw FASTQ seq files provided along with standard bioinformatics including differential expression analysis. $1025/sample
De novo Whole-Genome Sequencing, Human Whole-Genome Sequencing, ChIP-Sequencing and Methylation Sequencing, and Custom Targeted Sequencing please inquire for pricing (David Goerlitz, x7 -0832, dsg3@georgetown.edu)
   
DNA Sequencing and Fragment Analysis through GENEWIZ (click to see detailed fee structure and submission instructions)
DNA sequencing (reaction and sequencing) $5.00/reaction
DNA sequencing (sequencing only) $2.50/reaction
Fragment analysis (run only) $5.00/reaction
Fragment analysis (run and data analysis) $20.00/reaction
 
SNP Genotyping and CNV Analysis 
ABI
SNP genotyping by TaqMan real-time PCR $1.5/gene/sample + $60/plate
Illumina  
SNP genotyping by Illumina BeadXpress (Click to see detailed fee structure)
Qiagen pyrosequencing  
Tri-allelic SNP genotyping by pyrosequencing $5.00/sample
Affymetrix  
Genome-Wide Human SNP Array 6.0 Full Service (sample preparation, labeling, hybridization, scanning, and basic data analysis*) $365/sample (chip purchased separately)
Agilent   
Agilent array scan (hybridization, scanning, and basic data analysis*) $100/array (arrays purchased separately)
Agilent CGH array Full Service (sample preparation, labeling, hybridization, scanning, and basic data analysis*) $200/array (arrays purchased separately)
   
DNA Methylation Analysis 
Qiagen pyrosequencing
Validation and optimization of new assays (does not include inventoried assays) $500/assay
Methylation analysis by pyrosequencing includes bisulfite modification of DNA $16.50/sample
ABI
Real Time Methylation Specific PCR (RT-MSP) $1.5/gene/sample + $60/plate
Agilent   
Agilent array scan (hybridization, scanning, and basic data analysis*) $100/array (arrays purchased separately)
Agilent DNA Methylation array Full Service (sample preparation, labeling, hybridization, scanning, and basic data analysis*) $200/array (arrays purchased separately)
Illumina  
Illumina Infinium Human Methylation450 BeadChip Full Service (sample preparation, labeling, hybridization, scanning, and basic data analysis*) $450/array (includes cost of the array)
Methylation by Illumina BeadXpress 96-plex CpG: $12,902.40 +$250/plate (480 samples)
384-plex CpG: $44,102.40 +$250/plate (480 samples)
(Click to see detailed fee structure)
 
microRNA Expression Profiling
ABI  
miRNA analysis by TaqMan Low Density Array (TLDA) Full Service includes microRNA array, RT-PCR, PCR reaction, and basic data analysis* $550/sample (includes cost of the array)
Affymetrix  
GeneChip miRNA 2.0 array Full Service (sample preparation, labeling, hybridization, scanning, and basic data analysis*) $325/sample (chip purchased separately)
Agilent   
Agilent Human miRNA array Full Service (sample preparation, labeling, hybridization, scanning, and basic data analysis*) $350/sample (includes cost of the array)
   
RNA Quality Assessment
RNA quality measurement by Agilent Bioanalyzer $50/chip
 
DNA/RNA Isolation and Plating and Assay Preparation
DNA/RNA Isolation  $15/sample
Plating and Assay preparation $350/96-well plate (reformat 96 DNA
samples in tubes to 96-well deep  plate, OD and normalize)
$200/Reformat 4 96-well plates into 384-well plate, plus $6/384-well plate created
 
siRNA Screening  
siRNA Screen (initial consultation and experimental design support, liquid robotic handling in 96- and 384-well plate format) $2800/screen
 
User Operated Instruments
ABI Sequence Analyzer HT7900 $20/hr
NanoDrop ND-1000 Spectrophotometer $10/use (not to exceed $25/day, $375/year)
Berthold LB 96 Luminometer $25/use (not to exceed $25/day, $375/year)
Tecan Ultra Fluorescence Microplate Reader $25/use (not to exceed $25/day, $375/year)
Amaxa Nucleoporator $25/use (not to exceed $25/day, $375/year)
Axon GenePix 4000A Scanner $25/use (not to exceed $25/day, $375/year)
Alpha Innotech FluoroChem-8900 $25/use (not to exceed $25/day, $375/year)
Beckman DU-640 Spectrophotometer $25/use (not to exceed $25/day, $375/year)
BioTek EL-808IU-PC Microplate Reader $25/use (not to exceed $25/day, $375/year)
LI-COR Odyssey Infrared Imager $25/use (not to exceed $25/day, $375/year)
   
Advanced Data Analysis**
Avaliable software tools include: $75/hour
R/Bioconductor
Partek Genomics Suite
Agilent GeneSpring
Agilent DNA Analytics (Genomics Workbench)
Integromics RealTime StatMiner
Oncomine Research Premium Edition
Ingenuity Pathway Analysis (IPA) Tool
*Basic data analysis includes data pre-processing, fold change calculation, statistical significance analysis (p-values), and multiple testing correction (e.g., FDR)
**Advanced data analysis includes gene set enrichment analysis, cluster analysis (heatmaps), network/pathway analysis, principal component anlysis (PCA), etc.
If you have any questions regarding data analysis, please contact James Li (xl35@georgetown.edu or x7-8533)
8/20/13