Genomics and Epigenomics Shared Resource

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Services

Next-Generation Sequencing

GESR provides the following next-generation sequencing services:

 

Exome Sequencing

  • Exome Sequencing Full Service (DNA QC, whole-exome capture using the Agilent SureSelect kit v4 (54Mb), library prep, indexing and 100 bp paired-end reads on the Illumina HiSeq2000/2500 (50X coverage). Raw FASTQ seq files provided along with standard bioinformatics including reference genome mapping and variant calls.

RNA-Seq

  • RNA-Seq Full Service (RNA QC (Agilent Bioanalyzer), library preparation (Illumina TruSeq), indexing and 100 bp singleton reads on the Illumina HiSeq2000/2500 (20M reads/sample). Raw FASTQ seq files provided along with standard bioinformatics including differential expression analysis.

Custom Targeted Sequencing, de novo Whole-Genome Sequencing, Human Whole-Genome Sequencing, ChIP-Sequencing and Methylation Sequencing

For information on next-generation sequencing contact David Goerlitz. For data analysis, contact James Li Habtom Ressom.

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