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Biacore T-100 Technical Information

Established Applications for the Biacore Machines

The following are a list of established applications of the Biacore technology. Protocols are available for all of these applications upon request.

  • Characterization of monoclonal antibody epitope specificity using Biacore's SPR technology.

  • Comparison of a structural and functional binding domain in human growth hormone.

  • Multicomponent analysis: formation of a quarternary signal transduction complex.

  • The study of interactions between lectins and immobilized glycoproteins using Biacore's SPR technology.

  • Observing DNA at work with Biacore's SPR technology.

  • Ligand Fishing with Biacore 3000: Selective binding, recovery and identification by MALDI MS of a specific interaction partner.

  • The Determination of Clenbuterol Residues in Bovine Urine by Biacore's SPR Technology.

  • The Detection of Sulfonamides in Pig Bile and Edible Tissue.

  • Ligand immobilization using thiol-disulphide exchange.

  • SPR analysis of a trans-membrane receptor protein immobilized in a tethered lipid bilayer.

  • Kinetic and thermodynamic characterization of HIV-1 protease inhibitors using Biacore.

  • SPR characterization of lead compounds against CD80: Blocking T-cell activation in rheumatoid arthritis therapy.

  • Using small peptide nucleic acids (PNA) in a Biacore assay to detect disease-specific point mutations.

  • Unraveling the mechanisms of RNA-binding protein functions using SPR-based kinetic analysis.

  • Optimizing the DNA-binding characteristics of anti-cancer and anti-parasitic drugs.

  • Analysis of fibril elongation by Biacore's SPR technology.

  • Characterization of the membrane-binding properties of two peripheral proteins by SPR.

  • Initial studies towards automated screening for enzyme inhibitors by SPR-MS.

  • A Biacore-based assay for measuring the absorption of drugs to lipid membranes in early ADME studies.

  • Scaffold-class analysis of lead compounds by SPR using a combination of screening and kinetic characterization.

  • SPR-MS as a tool for quality control of recombinant proteins.

  • The detection of ractopamine residues in porcine urine and liver using a SPR biosensor.

  • Characterization of drug-plasma protein interactions using surface plasmon resonance.

  • Rapid development of a GMP-compliant Biacore assay for the determination of antibody concentration.

  • Detection of chloramphenicol and chloramphenicol glucuronide residues in poultry muscle, honey, milk and shellfish using SPR.

  • The detection of multi ß-agonist residues in liver using Biacore's SPR technology.

  • Detection of sulfonamides in porcine muscle using an SPR biosensor.

  • Validation of a Biacore concentration assay.

  • Rapid monoclonal antibody screening using automated SPR-based biosensors.

  • Optimizing SPR-MS for isolation and characterization of low level impurities in complex, protein pharmaceutical samples.

  • Characterization of a poxvirus-derived chemokine inhibitor: Using SPR in the study of viroceptor interactions with host ligands.

  • Biacore and biodefense: the generation and characterization of protective monoclonal antibodies to botulinum neurotoxin type A.

  • Classification of binding preferences for 1000 novel transcription factors synthesized using a wheat germ cell-free translation system.

  • Development of a Biacore assay using membrane proteins reconstituted in liposomes as analyte.

  • A novel assay to measure telomerase activity using SPR.

  • Development of immunotherapeutics and immunization regimes using Biacore T100.

  • Transition state thermodynamic analysis using Biacore T100.

  • Selection of protein therapeutic candidates using Biacore T100.