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Histopathology and Tissue Shared Resource

5. Imaging

Microscopy: The laboratory is equipped with:

• Olympus BX61 DSU Fluorescent scope with spinning disc confocal, equipped with digital cameras:
  • Brightfield
  • Immunofluorescence filters
    • FITC
    • Cy3
    • Cy5
    • DAPI
  • Z-stack
• Dual headed microscopes

• Scanning Qicam Q Imaging Camera on Olympus BX51 microscope for scanning full slides and TMAs

• ImageProPlus Software package for automated quantification of stained tissue sections

• Stereomicroscope: Olympus SZX12 with 0.5X and 1.2X objectives and zoom (0.7X-9.0) give high magnification for dissection and examination.
  • Equipped with Olympus DP12 digital camera for imaging
• Histo Rx microscope system for quantitative immunofluorescence imaging
  • Measure intensity of fluorescence for levels of protein expression
  • Measure subcellular localization