Flow Cytometry and Cell Sorting Shared Resource

Contact Information

Flow Cytometry and Cell Sorting
W304, Research Building

Director:
Karen Creswell, Ph.D.
Office: W308, Research Building
(202) 687-3772
creswelk@georgetown.edu

Instrument Operator:
(202) 687-2233

Description

The Lombardi Comprehensive Cancer Center Flow Cytometry and Cell Sorting Shared Resource provides flow cytometry and fluorescence activated cell sorting (FACS) in a variety of research applications at Georgetown University.

Karen Creswell, Ph.D., director of the facility, has extensive experience with flow cytometry and cell sorting including immunofluorescence methods, DNA histogram/cell cycle analysis, and sterile cell sorting, and is available for development of specific applications. A full-time instrument operator also provides for comprehensive support of this technology. Approximately 50 investigators use this facility annually.

Two instruments, a Becton Dickinson FACStar Plus dual laser system and a FACSort system, facilitate efficient utilization of this technology for routine, as well as more complex applications. Computer software is available for DNA cell cycle phase analysis as well as a variety of other multiparameter research applications. Both instruments are interfaced to the LCC computer network enabling investigators convenient access to their data for analysis and publication quality hardcopy.

Eligibility

All Georgetown University investigators may use the Flow Cytometry and Cell Sorting Shared Resource.

Procedures

New shared resource facility users must schedule their first experiment with Dr. Creswell. Thereafter, experiments may be scheduled by the instrument operator. Currently, it is necessary to schedule experiments one week in advance in order to obtain a specific day and time.

Fees

There is a per-hour charge for data acquisition and analysis, plus a set-up fee per experiment. Cancellations made less than one day in advance will be charged for one hour. Investigators are cautioned to be on time to avoid conflict with other scheduled experiments. A fee schedule can be obtained from the facility.

Acknowledgements

For funding purposes, it is important to acknowledge the Flow Cytometry Shared Resource in all publications that include data derived from the resource.