Cytogenetics Shared Resource Laboratory
Contact INFORMATION
Director: Jan K. Blancato, PhD, FACMG
Tel: (202) 444-1536
Fax: (202) 444-1770
E-mail: blancatj@georgetown.edu
Address:
M4000, Main Hospital
3800 Reservoir Rd NW
Washington DC 20007
General Information:
ma362@georgetown.edu
Description
The Cytogenetics Shared Resource Laboratory (CSRL) provides Lombardi
Comprehensive Cancer Center investigators and investigators from other institutions with a variety of cytogenetic analyses. CSRL performs both
classical and molecular cytogenetic studies on research specimens from
human and mouse tissues and cell lines.
Metaphase preparations on a variety of cell
types, e.g., blood, bone marrow, cell lines, and
solid tumors can be prepared. 
Classical cytogenetic studies offered by the
resource include traditional G-band karyotype
preparations for overall
screen of numerical and structural chromosome
abnormalities.
Molecular cytogenetic studies employ
Fluorescence In Situ Hybridization (FISH)
and can be performed on metaphases and/or
interphase cells from tissues and other sources.
Chromosome breakage analysis for evaluation
of chromosome damage to human and murine DNA is
performed.
Investigators are encouraged to contact the
director of CSRL to discuss development of
additional related assays as needed to
facilitate their research projects.
Services
- Cell culture and harvest
- G-band/inverted DAPI
- Karyotyping
- Chromosome breakage analysis
- FISH/Tissue FISH hybridization, click here to download a FISH request form.
- Mouse SKY
- Mouse ploidy analysis
- Consultation/discussion with LCCC
investigators regarding cytogenetic analysis
for potential research projects.
Please use a
request form to request services and
refer to the
fee list for individual service fees.
Fees listed are for LCCC members only. Non-LCCC investigators can contact the Director
for specific information.
To download our letter of support,
click here.
Selected Publications
Blancato J, Singh B, Liu A, Liao DJ,
Dickson RB. Correlation of
amplification and overexpression of the c-myc
oncogene in high-grade breast cancer: FISH,
in situ hybridization and
immunohistochemical analyses.
British Journal of Cancer
2004;90:1612-1619.
Click here to download PDF.
Coopman PJP, Do MTH, Barth M, et al.
The Syk tyrosine kinase suppresses malignant
growth of human breast cancer cells.
Nature 2000;406:742-747.
Click here to download PDF.
Acknowledgements
For funding purposes, it is important to acknowledge the Cytogenetic Shared
Resource in all publications that include data derived as a result of using the resource. Please
mention in the acknowledgement that the Cytogenetic Shared Resource Laboratory is partially supported by NIH/NCI grant 1P30-CA-51009 (Cancer Center Support Grant, to
Lombardi Comprehensive Cancer Center).
