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BMI Description

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Equipment

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Services

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Billing

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Forms

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Biacore Molecular Interaction Shared Resource

Contact Information

Director: Aykut Üren, M.D.
Assistant Professor
Georgetown University Medical Center
Lombardi Cancer Center
Research Building, Room: E312
3970 Reservoir Road, N.W.
Box 571469
Washington, D.C. 20057-1469

Phone: 202 687 9504
Fax: 202 687 1434
e-mail: au26@georgetown.edu

Description

The Biacore Molecular Interaction (BMI) core facility located in Research Building room E312 will provide customized biomolecular analysis services to Georgetown University faculty and staff.  The two Biacore machines in our facility utilize the Surface Plasmon Resonance (SPR) technology to study biomolecular binding events on a chip surface.  The basic principle involves immobilization of a ligand on a sensor chip followed by delivery of an analyte by a microfluidic system.  Any protein, DNA, RNA, lipid, carbohydrate, polysaccharide, cell, virus, drug, drug like molecule (organic or inorganic) can be used as the ligand or analyte.  Since the detection system is based on measuring total mass on the sensor chip surface neither ligand nor analyte has to be tagged.  In addition to identifying binding partners to a target molecule, SPR also provides quantitative data on:

  • Specificity: How specific is the binding between two molecules?

  • Concentration: How much of a given molecule is present and active?

  • Kinetics: What is the rate of association and dissociation?

  • Affinity: How strong is the binding?

Equipment & Billing

There are two Biacore instruments available for users. 

Biacore Equipment
The Biacore 1000 and T-100

Learn about the applications of the machines.

Read the T-100 Technical Information.

Instrument and technician time for Biacore 1000 and Biacore T-100 are $10/hour and 15/hour respectively.  There is a $300 set-up fee for new experimental approaches.  Set-up fee will cover the machine and technician time during the initial period for characterizing optimum immobilization and binding conditions. 

Biacore Chips

Users can purchase sensor chips directly from Biacore or BMI can order them for the user for the same price.  These fees are for LCCC members only.  Please check the sample submission forms for non-LCCC fees.

Forms

Sample submission forms for: T-100 B-1000

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