The following are a list of established applications of the Biacore technology. Protocols are available for all of these applications upon request.
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Characterization of monoclonal antibody epitope specificity using Biacore's SPR technology.
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Comparison of a structural and functional binding domain in human growth hormone.
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Multicomponent analysis: formation of a quarternary signal transduction complex.
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The study of interactions between lectins and immobilized glycoproteins using Biacore's SPR technology.
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Observing DNA at work with Biacore's SPR technology.
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Ligand Fishing with Biacore 3000: Selective binding, recovery and identification by MALDI MS of a specific interaction partner.
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The Determination of Clenbuterol Residues in Bovine Urine by Biacore's SPR Technology.
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The Detection of Sulfonamides in Pig Bile and Edible Tissue.
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Ligand immobilization using thiol-disulphide exchange.
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SPR analysis of a trans-membrane receptor protein immobilized in a tethered lipid bilayer.
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Kinetic and thermodynamic characterization of HIV-1 protease inhibitors using Biacore.
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SPR characterization of lead compounds against CD80: Blocking T-cell activation in rheumatoid arthritis therapy.
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Using small peptide nucleic acids (PNA) in a Biacore assay to detect disease-specific point mutations.
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Unraveling the mechanisms of RNA-binding protein functions using SPR-based kinetic analysis.
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Optimizing the DNA-binding characteristics of anti-cancer and anti-parasitic drugs.
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Analysis of fibril elongation by Biacore's SPR technology.
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Characterization of the membrane-binding properties of two peripheral proteins by SPR.
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Initial studies towards automated screening for enzyme inhibitors by SPR-MS.
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A Biacore-based assay for measuring the absorption of drugs to lipid membranes in early ADME studies.
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Scaffold-class analysis of lead compounds by SPR using a combination of screening and kinetic characterization.
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SPR-MS as a tool for quality control of recombinant proteins.
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The detection of ractopamine residues in porcine urine and liver using a SPR biosensor.
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Characterization of drug-plasma protein interactions using surface plasmon resonance.
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Rapid development of a GMP-compliant Biacore assay for the determination of antibody concentration.
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Detection of chloramphenicol and chloramphenicol glucuronide residues in poultry muscle, honey, milk and shellfish using SPR.
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The detection of multi ß -agonist residues in liver using Biacore’s SPR technology.
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Detection of sulfonamides in porcine muscle using an SPR biosensor.
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Validation of a Biacore concentration assay.
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Rapid monoclonal antibody screening using automated SPR-based biosensors.
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Optimizing SPR-MS for isolation and characterization of low level impurities in complex, protein pharmaceutical samples.
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Characterization of a poxvirus-derived chemokine inhibitor: Using SPR in the study of viroceptor interactions with host ligands.
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Biacore and biodefense: the generation and characterization of protective monoclonal antibodies to botulinum neurotoxin type A.
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Classification of binding preferences for 1000 novel transcription factors synthesized using a wheat germ cell-free translation system.
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Development of a Biacore assay using membrane proteins reconstituted in liposomes as analyte.
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A novel assay to measure telomerase activity using SPR.
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Development of immunotherapeutics and immunization regimes using Biacore T100.
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Transition state thermodynamic analysis using Biacore T100.
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Selection of protein therapeutic candidates using Biacore T100.
