Tissue Culture Shared Resource

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Contact Information

Director: Bassem Haddad, MD
Telephone: (202) 444-0759
haddadb1@georgetown.edu
Lombardi Building LL S-111

Manager: Dionnyssia Clagett, MS
Telephone: (202) 687-0240
dac58@georgetown.edu
Research Building Suite W314

Address:
New Research Building W314
Georgetown University
3970 Reservoir Rd NW
Washington DC 20057-1468

Description

The Tissue Culture Shared Resource (TCSR) exists to assist the Cancer Center investigators and other members of the Georgetown University research community with all the tissue culture related aspects of their research and to maintain the Lombardi cell line repository. To this end the Resource provides consultation, facilities and services to a diverse group of investigators involved in cancer research.

Specifically, the shared resource:

  • Maintains the Lombardi cell line repository and provides frozen vials or live cultures of these lines to investigators.
  • Equips, monitors, stocks and services four separate shared tissue culture laboratories for the use of investigators who do not have facilities to perform tissue culture in their own laboratories or who need additional tissue culture space.
  • Prepares, tests and supplies tissue culture reagents of the highest quality, that have been specifically tested for suitability in the relevant systems, as inexpensively as possible.
  • Provides mycoplasma testing and genetic fingerprinting of cells.
  • Provides a B-cell transformation service to facilitate cancer genetics studies.
  • Provides DNA preparation
  • Assists with the establishment of primary cultures
  • Provides large scale tissue culture services
  • Provides a safe, secure cryo-storage facility for investigators to keep frozen stocks of their cancer cell lines.
  • Provides expert consultation regarding the use of tissue culture techniques in cancer research and provides training to investigators at all levels in the use of this technology.

Acknowledgements

For funding purposes, it is important to acknowledge the Tissue Culture Shared Resource (TCSR) in all publications that include data derived as a result of using the resource. Please mention in the acknowledgement that the TCSR is partially supported by NIH/NCI grant 1P30-CA-51008 (Cancer Center Support Grant, to Lombardi Comprehensive Cancer Center).