Microscopy and Imaging Shared Resource

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Educational Resources

Fluorescence Tutorials (From Molecular Probes)

• Introduction to Fluorescence  - Audio link, need speakers

• Fluorescence Spectra - Audio link, need speakers

• Filters and Light Sources - Audio link, need speakers

Basic Microscopy (From Olympus)

• Introduction

• The Concept of Magnification

  • Interactive Tutorial (Simple Magnification)

• Introduction to Lenses and Geometrical Optics

• Microscope Illumination

  • Phase or DIC?
  • Köhler Illumination

• Microscope Objectives

  • Numerical Aperture and Resolution
  • Why do we use Oil? (From Cargille Labs)

   

Advanced Microscopy (From Olympus, Nikon and Molecular Expressions)

• Nikon review articles on imaging methods

• Laser Scanning Confocal Microscopy

• Spinning disk

• Total Internal Reflection Fluorescence (TIRF) Microscopy

• Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Loss after Photobleaching (FLIP)

  • Olympus FRAP / FLIP

• Fluorescence Resonance Energy Transfer (FRET) Microscopy

  • FRET Pair Spectra (From the University of Virginia)
  • Zeiss
  • Olympus
  • FLIM

• Multiphoton Fluorescence Microscopy

  • Zeiss “Applications and Techniques Multiphoton”
  • Chamleon XR laser specifications
• Multispectral imaging
  • Zeiss Spectral Imaging and Emission Fingerprinting
  • Nuance Multispectral Imaging

 

Image Analysis

• Metamorph (From Molecular Devices)

• ImageJ - Free software for Image Analysis (From NIH)

• Volocity

 

Fluorophore Database

• Bio-Rad Fluorophore Database (From Nexus Solutions)

• Fluorescent Spectra Interactive Database (Univ. of Arizona)

 
Live Cell Imaging Equipment Resources

• Bioptechs--Instructions for Delta T Open Dish System

• Bioptechs--Instructions for FCS2 Closed Chamber System

• Bioptechs--Instructions for Objective Heater System

• Bioptechs--Instructions for Objective Wrench & Pliers

• Bioptechs--Air-therm heater information

 

Gallery
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