Microscopy and Imaging Shared Resource

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Olympus AH2 Vanox Bright Field Microscope System

Olympus AH2Olympus AH2 Microscope


Objectives:

  • Plan, 2.5X, N.A. 0.08

  • Plan, 6.3X, N.A. 0.16

  • S Plan Apo, 10X, N.A. 0.40

  • S Plan, 20X, N.A. 0.46

  • Plan Neofluor, 25X oil, water, glycerin, N.A. 0.8

  • S Plan Apo, 40X, N.A. 0.95

  • Plan Apo, 63X oil, N.A. 1.4

  • S Plan, 100X oil, N.A. 1.25

Filter Cubes:

  • DAPI: (EX=360nm, DM=400nm, EM=460nm)

  • FITC: EX =480nm, DM =505nm, EM =535nm)

  • RhodamineTRed: (EX =545nm, DM =570nm, EM =572nm)/ (EX=560nm, DM =595nm, EM =645nm)

  • Nomarski

  • Dark field optics

Camera:

  • QImaging micropublisher 5.0RTV digital camera

Computer & Imaging Software:

  • 3.0 GHz Pentium IV Windows XP, CD/DVD/USB1 data transmission

  • MetaMorph image aquisition and analysis software (version 6.3r7)

Acknowledgments:
PLEASE REMEMBER: For funding purposes, it is important to acknowledge the Microscopy & Imaging Shared Resource Facility in all publications that include data derived from the Facility. For Olympus Vanox AH2 microscope, this should include the following statement:

"This work was supported in part by the Lombardi Comprehensive Cancer Center Microscopy and Imaging Shared Resource funded by U.S. Public Health Service Grant 2P30-CA-51008, 1S10 RR15768-01, 1 S10 RR019291-01A2, and the Carey Lackman Slease Fund."

If you have comments or suggestions, email Michael Johnson, director of MISR at Johnsom@georgetown.edu.

For mounting medium website, go to:
http://www.emsdiasum.com/microscopy/products/chemicals/adhesive.aspx#17985